Exam pattern, Syllabus for APPSC Biotechnology Degree Lecturers 2017

Exam pattern, Syllabus for APPSC Biotechnology subject Degree Lecturers 2017 in Government Degree Colleges in A.P. Collegiate: APPSC has given the Degree College Lecturers Recruitment 2017 notification and online applications are invited online from qualified candidates to the post of Degree College Lecturers in in Govt Degree Colleges in the State of Andhra Pradesh. The proforma Application will be available on Commission’s Website (www.psc.ap.gov.in) from 29/12/2016 to 28/01/2017 (Note: 27/01/2017 is the last date for payment of fee up- to 11:59 mid night). APPSC Degree College Lecturers Recruitment 2017 notification no.26/2016 and apply online now @ http://appscapplications17.apspsc.gov.in/

Scheme of Exam:
PART-A: Written ‘Examination (Objective Type)
Papers No. of Questions Duration (Minutes) Maximum Marks
Paper-1: General Studies & Mental Ability 150 150 150
Paper-2:  Biotechnology subject 150 150 300
PART-B: Interview (Oral Test) 50
NEGATIVE  MARKS: As per G.O.Ms. No.235, Finance (HR-I, Plg & Policy) Dept., Dt. 06/12/2016, for each wrong answer will be penalized with 1/3rd of the marks prescribed for the question.

READ | APPSC Degree Lecturers Recruitment

Biotechnology Subject Syllabus:
CELL BIOLOGY: Diversity of cell size and shape. Cell theory, microscopic techniques for study of cells. Sub-cellular fractionation and criteria of functional integrity. Cellular organelles-Plasma membrane, cell wall, Mitochondria, Chloroplast, Nucleus and other organelles and their organization, structure and functions. Cell motility-cilla, flagella of eukaryotes. Transport of nutrients, ions and macromolecules across membranes. Liposomes, drug delivery systems, cellular energy transactions- role of mitochondria and chloroplast. Molecular assemblies like membranes; structure and functional aspects. Ribosome’s, extra cellular matrix. Cell cycle – Overview of eukaryotic cell cycle, regulation of cell cycle by cell growth and extra cellular signals. Cell cycle check points. Regulators of cell cycle progression – MPF, cyclins and cyclin-dependent kinases. Cell death and proliferation – Apoptosis: definition, differences between apoptosis and necrosis and mechanism. Cancer: Types and Classification, Development and Properties of Cancer cells. Somatic

mutations in cancer cells. Biotic and abiotic stress in plants. Signal transduction: types of receptors, second messengers (calcium, phoshoinositides and Nitric oxide). Meiosis, Gametogenesis, fertilization and Development of chick embryo. BIOMOLECULES AND ANALYTICAL TECHNIQUES: - Chemical foundations of Biology water, pH, pK, acids, bases, buffers, weak bonds, covalent bonds. Principles of thermodynamics. Classification, structure and functions of carbohydrates, amino acids, proteins, nucleic acids and lipids. Chromatography Methods; partition, ion exchange, and affinity methods, criteria for purity, proteins and nucleic acids sequencing methods, Hormones, vitamins and minerals.
Analytical techniques: Principle, instrumentation and applications of VIS/UV, IR, NMR, LASER Raman Spectroscopy MASS Spectroscopy, Fluorescence Spectroscopy, Differential colorimetry, X-ray Crystallography, Ultra centrifugation, Electron Cryomicroscopy and Scanning Tunneling microscopy. Methods of cell study; confocal microscopy, Flowcytometry and FACS (fluorescence activated cell sorter) and atomic force microscopy. Radiochemical methods; Stable and radioactive isotopes, measurement of radioactivity by Liquid scintillation counting, GM counters and autoradiography. Specific activity and precursor-product relationship. Tracer studies and Effect of radiation on cells.
MOLECULAR BIOLOGY: - DNA Structure, replication, repair and recombination, Transcripition, regulation and post transcriptional modifications in Prokaryotes and Eukaryotes. Transcriptional and post-transcriptional gene silencing. Translation and regulation in Prokaryotes and eukaryotes, co-translation and post- translational modifications of proteins. Protein Localization-Synthesis of secretory and membrane proteins, import into nucleus, mitochondria, chloroplast and peroxisomes, Biology of Cancer-Oncogenes and Tumour Suppressor genes, Structure, function and mechanism of action of pRB and p53 tumor suppressor proteins.
Antisense oligonucelotides, molecular targets of drug action and Ribozyme Technology. Homologous Recombination-Holliday model gene targeting, gene disruption, FLP/FRT and Cre/Lox recombination RecA and other recombinases. Molecular Mapping of Genome, Genes, mutation and mutagenesis, site directed mutagenesis and Human genome project, Transposons

BIOSTATISTICS: - Frequency distribution, Distribution of data bionomial, poission and normal. Measures of central tendency-mean, median, mode and standard deviation-probability distribution-regression-correlation- Analysis of variance-tests of significance- T-test, F-test, Chi-square test.

MICROBIOLOGY: - Discovery of the microbial world; Distinguishing features of prokaryotes and eukaryotes; general role of microorganisms in transformation of organic matter and in the causation of diseases; Microbial taxonomy; Classification, Nomenclature and new approaches to microbial taxonomy; Pure culture techniques; sterilization methods; Principles of microbial nutrition and composition of culture media; culture enrichment techniques; Growth and its mathematical expression; synchronized cultures, Culture collection and maintenance of cultures; Purple and green bacteria Ricketsias; Chlamydia and Mycoplasma. Archea; Viruses: structure and replication of viruses; DNA viruses and RNA viruses; Viroids and Prions; Viruse and their Genetic System; Bacteriophages; RNA phages; Retroviruses, Biomass and Bioenergy.

IMMUNOLOGY:- Phylogeny of immune System; Innate and acquired immunity; Hematopoietic and differentiation, cells and organs of the immune system; Lymphocyte trafficking; Antigencity and super antigens; Immunoglobulin types, structure and function, Antigen-antibody interactions; Blood groups, Cell migration and Homing, Immunoglobulin and gene organization. Major histocompatibility complex, BCR and TCR and generation of diversity; Complement system, Antigen processing and presentation, generation of humeral and cell mediated immune responses; Activation of B-and T- lymphocytes, Cytokines and their role in immune regulation; Cell mediated cytotoxic, Hypersensitivity, Autoimmunity, Transplantation, Tumor Immunology, AIDS and other Immunodeficiency; Hybridoma Technology, Psychoneuro-immunology,
Single chain antibodies, theories of antibody diversity, Vaccines – Concept of immunization, routes of vaccination. Types of vaccines – Whole organism (attenuated and inactivated) and component vaccines (synthetic peptides, DNA vaccines, recombinant vaccines, subunit vaccines, conjugate vaccines. Vaccine delivery systems.

BIOPROCESS ENGINEERING:- Fermentation-types of fermentors and bioreactor design, cell concentration and stirring. Filtration, methods of cell disruption. Downstream processing, industrial applications of bioprocess. Synchronized and continuous culturing. Industrial production of glutamic acid, citric acid, ethanol, penicillin and riboflavin. Purification and crystallization of products.

ENZYME TECHNOLOGY:- Discovery classifications and nomenclature of enzymes. Techniques of enzymes isolation and assay, Intracellular localization of enzymes, Isoenzymes, Multienzyme complexes and multifunctional enzymes Physico-chemical characterization of enzymes, Enzymes kinetics, kinetic of enzymes of inhibition. Allosterism, Enzyme memory and mnemonic enzymes, Various techniques used for the immobilization of enzymes and their applications in Biotechnology. Purification of enzymes and their applications, Single cell proteins. Industrial application of enzyme, applications in biosensors.

ENVIRONMENTAL BIOTECHNOLOGY:- Ecological balance, resiliency of ecosystem and sustainable development, environmental pollution and global problems, water, air, soil pollution and their impacts on environment and biotechnological approaches for management, waste water treatment: aerobic and anaerobic processes, bioremediation of contaminated soils and waste land, biotechnological treatment for industrial effluents and solid wastes. GM microbes GENETIC ENGINEERING:- Discovery, properties and application of Restriction enzymes, Cloning and expression vectors, Purification of plasmids, genomic DNA and mRNA. Genomic and cDNA Library construction and screening of recombinants by hybridization methods, Reporter assays, protein engineering- site directed mutagenesis, adding disulfide bonds – changing asparagines to other amino acids modification of metal cofactor requirements. Increasing of specific activity Stability to thermal and salinity conditions, Phage Display library and yeast two hybrid system. Gene transfer methods Tagging, Role of gene tagging analysis; Gene Therapy, Gene silencing methods, Biochips.

BIOINFORMATICS-:- Biological databases, ORF finding, EST analysis, gene identification, microsatellite repeat patterns, Blast all flavours, Mutation matrix, global Vs local alignments, Dot plots, PAM and BLOSUM matrices, Multiple sequence modeling, alignments dendrograms, phylograms, protein structure prediction methods, molecular modeling, Primer design, QSAR, Drug designing

PLANT BIOTECHNOLOGY:- Selection of explants, micro and macro Propagation techniques in plant tissue culture suspension culture, single cell. Anther, pollen and ovary culture for production of haploid plants. Cryopreservation for germplasm conservation. Plant Transformation technology, Transgene stability and gene silencing. Application of plant Transformation for productivity and performance. Metabolic Engineering and Industrial products: Plant secondary metabolites, industrial enzymes, biodegradable plastics, therapeutic proteins, antibodies, edible vaccines. Molecular marker assisted selection and Breeding: RFLP maps, RAPD markers, STS, microsatellites, SCAR (sequence characterized amplified regions), SSCP (single stand conformational polymorphism), AFLP, GM Crops

ANIMAL BIOTECHNOLOGY: - Animal cell culture technology, simple and complex growth media, cell culture techniques, primary and established cell lines. Biology and characterization of the cultured cells, measuring growth parameters, maintenance of cell culture, Measurement of viability and cytotoxicity, cell separation. Scaling–up of animal cell culture. Cell synchronization. Cell cloning and micromanipulation. Cell transformation. Stem cell cultures, embryonic stem cells and their applications. Cell culture based vaccines. Organ and histotypic cultures. Apoptosis, measurement of cell death. Biodegradation of Toxicants, Diagnostic aids, organ perfusion studies., GM animals.
Principles and preparation of DNA and RNA probes and their applications: Study and expression of cloned genes in prokaryotes and eukaryotic systems. Microbial production of interferon, human growth hormone, insulin in E.coli. Genetic Engineering – Social and moral implications, national and international guidelines/regulations. Biotechnology patents and safeguarding human health.
Exam pattern, Syllabus, Instructions for APPSC Degree Lecturers 2017
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